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ccd 112con atcc crl  (ATCC)


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    ATCC ccd 112con atcc crl
    Ccd 112con Atcc Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccd+112con+atcc+crl/10__1016_slash_j__isci__2026__115193-509-210-211?v=ATCC
    Average 95 stars, based on 144 article reviews
    ccd 112con atcc crl - by Bioz Stars, 2026-07
    95/100 stars

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    LINC00858 expression in CRC tumor tissues and its clinical significance. (A) Expression level of LINC00858 in CRC tissues (n=60) compared with adjacent normal tissues (n=60) was assessed using qPCR. (B) Kaplan-Meier curves for overall survival in patients with CRC with high (n=30) and low LINC00858 expression level (n=30). (C) LINC00858 expression was analyzed using reverse transcription-qPCR analysis in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic fibroblast (CCD-112CoN). Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; q, quantitative; WT, wild-type.

    Journal: Oncology Reports

    Article Title: Long non-coding RNA LINC00858 promotes TP53-wild-type colorectal cancer progression by regulating the microRNA-25-3p/SMAD7 axis

    doi: 10.3892/or.2020.7506

    Figure Lengend Snippet: LINC00858 expression in CRC tumor tissues and its clinical significance. (A) Expression level of LINC00858 in CRC tissues (n=60) compared with adjacent normal tissues (n=60) was assessed using qPCR. (B) Kaplan-Meier curves for overall survival in patients with CRC with high (n=30) and low LINC00858 expression level (n=30). (C) LINC00858 expression was analyzed using reverse transcription-qPCR analysis in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic fibroblast (CCD-112CoN). Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; q, quantitative; WT, wild-type.

    Article Snippet: The human TP53-WT CRC cell lines, RKO, HCT116, Colo-678, LoVo, SW48, LS174T and human colonic fibroblast, CCD-112CoN were purchased from American Type Culture Collection ( ).

    Techniques: Expressing, Reverse Transcription

    miR-25-3p is a direct target of LINC00858. (A) Potential binding region between miR-25-3p and LINC00858 was predicted using StarBase V2.0 database. (B) miR-25-3p was validated as a direct target of LINC00858 using dual luciferase reporter assays in (B) SW48 and (C) RKO cells. (D) RIP assay confirmed the interaction between miR-25-3p and LINC00858. (E) The expression levels of miR-25-3p in CRC tissues (n=60) compared with that in adjacent normal tissues (n=60) were assessed using qPCR. (F) Correlation between LINC00858 and miR-25-3p expression levels was analyzed using Pearson's correlation analysis. (G) The expression levels of miR-25-3p in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic fibroblast (CCD-112CoN) were assessed using qPCR. (H and I) The expression levels of miR-25-3p in (H) SW48 and (I) RKO cells transfected with LINC00858 shRNAs. Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; miR, microRNA; q, quantitative; WT, wild-type; sh, short hairpin; MUT, mutant; AGO2, protein argonaute-2.

    Journal: Oncology Reports

    Article Title: Long non-coding RNA LINC00858 promotes TP53-wild-type colorectal cancer progression by regulating the microRNA-25-3p/SMAD7 axis

    doi: 10.3892/or.2020.7506

    Figure Lengend Snippet: miR-25-3p is a direct target of LINC00858. (A) Potential binding region between miR-25-3p and LINC00858 was predicted using StarBase V2.0 database. (B) miR-25-3p was validated as a direct target of LINC00858 using dual luciferase reporter assays in (B) SW48 and (C) RKO cells. (D) RIP assay confirmed the interaction between miR-25-3p and LINC00858. (E) The expression levels of miR-25-3p in CRC tissues (n=60) compared with that in adjacent normal tissues (n=60) were assessed using qPCR. (F) Correlation between LINC00858 and miR-25-3p expression levels was analyzed using Pearson's correlation analysis. (G) The expression levels of miR-25-3p in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic fibroblast (CCD-112CoN) were assessed using qPCR. (H and I) The expression levels of miR-25-3p in (H) SW48 and (I) RKO cells transfected with LINC00858 shRNAs. Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; miR, microRNA; q, quantitative; WT, wild-type; sh, short hairpin; MUT, mutant; AGO2, protein argonaute-2.

    Article Snippet: The human TP53-WT CRC cell lines, RKO, HCT116, Colo-678, LoVo, SW48, LS174T and human colonic fibroblast, CCD-112CoN were purchased from American Type Culture Collection ( ).

    Techniques: Binding Assay, Luciferase, Expressing, Transfection, Mutagenesis